isolation and genotyping of acanthamoeba strains from environmental sources in ahvaz city, khuzestan province, southern iran
نویسندگان
چکیده
b ackground: acanthamoeba spp . are free-living amoebae commonly found in the environmental sources such as water, soil, and air. this ubiquitous amoeba is the causative agent of amoebic kerati- tis (ak). the objective of the present study was to investigate the presence of acanthamoeba spp. in water and soil sources in ahvaz city, khuzestan province, southern iran. m ethods: in general, 110 samples of water and soil were taken from different localities of ahvaz including agricultural canals, rivers, and swimming pools. filtration and cultivation were carried out on non-nutrient agar medium (nna). axenic cultivation was performed for all of positive isolates. pcr analysis was conducted on positive samples. sequencing was done for 15 pcr products. geno - types were identified by blast search and homology analysis. r esult: acanthamoeba spp. was found in 43 (71.6%) of samples of water and 13 (26%) soil samples. genotyping of 15 samples proved that acanthamoeba belonged to t4 (86.6%), t2 (6.6%), and t5 (6.6%) genotypes. c onclusion: tyi-s-33 medium could be better than pyg medium for acanthamoeba axenic culture.
منابع مشابه
Isolation and Genotyping of Acanthamoeba Strains from Environmental Sources in Ahvaz City, Khuzestan Province, Southern Iran
BACKGROUND Acanthamoeba spp. are free-living amoebae commonly found in the environmental sources such as water, soil, and air. This ubiquitous amoeba is the causative agent of amoebic keratitis (AK). The objective of the present study was to investigate the presence of Acanthamoeba spp. in water and soil sources in Ahvaz City, Khuzestan Province, southern Iran. METHODS In general, 110 samples...
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عنوان ژورنال:
iranian journal of parasitologyجلد ۷، شماره ۴، صفحات ۲۲-۲۶
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